Journal
/
/
Isolering av murina retinala endotelceller för nästa generations sekvensering
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
DOI:

09:59 min

October 11, 2021

, , ,

Chapters

  • 00:04Introduction
  • 00:38Obtain Mouse Eyes for Retinal Isolation and Isolate Mouse Retinal Tissue
  • 02:30Digest Retinal Tissue into a Single Cell Suspension and Immunostain the Cells with Antibodies
  • 04:07Prepare for Fluorescence‐Activated Cell Sorting
  • 05:19Isolate Viable Endothelial Cells via FACS
  • 07:18Results: Analysis of Isolated Endothelial Cells and Next‐Generation Sequencing After Isolation
  • 09:03Conclusion

Summary

Automatic Translation

Detta protokoll beskriver en metod för isolering av murina postnatala retinala endotelceller optimerade för cellutbyte, renhet och livskraft. Dessa celler är lämpliga för nästa generations sekvenseringsmetoder.

Related Videos

Read Article