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Immunology and Infection

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JoVE Journal Immunology and Infection
Chemische cartografie benaderingen om trypanosomatide infectie te bestuderen
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Chemische cartografie benaderingen om trypanosomatide infectie te bestuderen

Article DOI: 10.3791/63255-v • 08:21 min • January 21st, 2022
January 21st, 2022 •
*1,2, *2,3, 1, 1,2,4
1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, 3Department of Anthropology, University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology, University of Oklahoma, Norman
* These authors contributed equally

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Dit protocol beschrijft de stappen om een 3D-model van metabolietverdeling tijdens trypanosomatide-infectie te genereren, inclusief monsterverzameling, metabolietextractie, een overzicht van vloeistofchromatografie-tandem massaspectrometriegegevensverzameling, 3D-modelgeneratie en ten slotte gegevensvisualisatie.

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Immunologie en infectie Nummer 179 Chemische cartografie metabolietverdeling ruimtelijke metabolomica 3D-modellen vloeistofchromatografie-tandem massaspectrometrie trypanosomatiden infectie tropisme
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