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Journal / Immunology and Infection / Kemiska kartografimetoder för att studera…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Kemiska kartografimetoder för att studera trypanosomatidinfektion
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Kemiska kartografimetoder för att studera trypanosomatidinfektion

Article DOI: 10.3791/63255-v • 08:21 min • January 21st, 2022
January 21st, 2022 •
*1,2, *2,3, 1, 1,2,4
1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, 3Department of Anthropology, University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology, University of Oklahoma, Norman
* These authors contributed equally

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Detta protokoll beskriver stegen för att generera en 3D-modell av metabolitfördelning under trypanosomatidinfektion, inklusive provsamling, metabolitutvinning, en översikt över vätskekromatografi-tandem masspektrometridataförvärv, 3D-modellgenerering och slutligen datavisualisering.

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Immunologi och infektion nummer 179 Kemisk kartografi metabolitfördelning rumslig metabolomik 3D-modeller vätskekromatografi-tandem masspektrometri trypanosomatider infektion trotropism
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