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Journal
/
Biology
/
非放射性鉄同位体を用いたマウス胎盤
を
横切る鉄輸送の定量
JoVE Journal
Biology
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JoVE Journal
Biology
Quantitating Iron Transport Across the Mouse Placenta
In Vivo
Using Nonradioactive Iron Isotopes
Please note that all translations are automatically generated.
Click here for the English version.
非放射性鉄同位体を用いたマウス胎盤
を
横切る鉄輸送の定量
DOI:
10.3791/63378-v
•
08:45 min
•
May 10, 2022
•
Veena Sangkhae
,
Elizabeta Nemeth
1
Center for Iron Disorders, David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:04
Introduction
00:37
Preparation of
58
Fe‐Transferrin
03:37
Administration of <sup58Fe‐Transferrin Intravenously to E17.5 Pregnant Mice
05:53
Tissue Processing for Quantitative Iron Analysis by Inductively Coupled Plasma Mass Spectrometry (ICP‐MS)
07:14
Results: Physiological Regulation of Mouse Placental Ferroportin During Maternal Iron Deficiency
08:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
この記事では、マウス妊娠中の鉄輸送の研究のために、トランスフェリン結合非放射性同位体鉄を調製および投与する方法を示します。胎児胎盤区画中の同位体鉄を定量するためのアプローチも記載されている。
Tags
Iron Transport
Mouse Placenta
Nonradioactive Iron Isotopes
Stable Iron Isotopes
Iron Uptake
Iron Regulation
Iron Distribution
Ferric Chloride
Ferric Nitrilotriacetate
Apotransferrin
Transferrin Loading
Molecular Weight Cutoff Column
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