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직렬 섹션 전자 현미경을 사용하여 간세포에서 소기관 간 접촉 부위의 입체 특성화
JoVE Journal
Biology
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JoVE Journal Biology
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
DOI:

09:12 min

June 09, 2022

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Chapters

  • 00:05Introduction
  • 01:08Trimming and Serial Sectioning of Embedded Samples
  • 04:12Grid Staining
  • 05:26Imaging Acquisition by TEM
  • 06:52Segmentation and 3D Reconstruction
  • 07:31Results: Segmentation and 3D Reconstruction Reviewing the Morphology of Endoplasmic Reticulum and the Associated ER-Mitochondria Contacts
  • 08:34Conclusion

Summary

Automatic Translation

간세포의 소기관 또는 다른 조직의 세포 사이의 막 접촉 부위의 입체 세부 사항을 획득하는 간단하고 포괄적 인 프로토콜.

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