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Estandarización y mantenimiento de cultivos de organoides hepáticos e intestinales caninos en 3D para su uso en investigación biomédica
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Estandarización y mantenimiento de cultivos de organoides hepáticos e intestinales caninos en 3D para su uso en investigación biomédica

Article DOI: 10.3791/63515-v • 08:39 min • January 31st, 2022
January 31st, 2022 •
1, 1, 2, 3, 2, 2, 4, 4, 1,3, 2,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology, Baylor College of Medicine

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Se describen métodos experimentales para cosechar células madre adultas de tejidos intestinales y hepáticos caninos para establecer cultivos de organoides en 3D. Además, se discuten las técnicas de laboratorio para garantizar un crecimiento constante y proporcionar procedimientos operativos estándar para cosechar, biobancar y revivir cultivos de organoides intestinales y hepáticos caninos.

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Medicina Número 179 Organoide perro canino células madre hígado intestino One Health estandarización
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