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Biology
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肝组织3D细胞培养模型中组蛋白翻译后修饰的全球水平定量
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Biology
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JoVE Journal
Biology
Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
Please note that all translations are automatically generated.
Click here for the English version.
肝组织3D细胞培养模型中组蛋白翻译后修饰的全球水平定量
DOI:
10.3791/63606-v
•
08:12 min
•
May 05, 2022
•
Jazmine-Saskya N. Joseph-Chowdhury
,
Stephanie Stransky
,
Sarah Graff
,
Ronald Cutler
,
Dejauwne Young
,
Julie S. Kim
,
Carlos Madrid-Aliste
,
Jennifer T. Aguilan
,
Edward Nieves
,
Yan Sun
,
Edwin J. Yoo
,
Simone Sidoli
1
Department of Biochemistry
,
Albert Einstein College of Medicine
Chapters
00:04
Introduction
00:53
Preparation of the 3D Culture System
02:20
Growth of Spheroids in the Bioreactor
03:15
Histone Extraction
04:27
The First Round of Derivatization
05:03
Desalting, Sample Cleanup, and Sample Preparation for LC-MS/MS
06:08
Results: Identification and Quantification of Histone Post-Translational Modifications
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议概述了如何使用三维细胞培养系统在接近生理状态下对染色质修饰进行建模,治疗和分析。
Tags
3D Cell Culture
Histone Post-translational Modifications
Liquid Chromatography Mass Spectrometry
Spheroid Formation
Bioreactor
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