Journal
/
Biology
/
Visualizing Bacterial Motility Based on a Color Reaction
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Visualizing Bacterial Motility Based on a Color Reaction

Visualizing Bacterial Motility Based on a Color Reaction

DOI:
10.3791/63706-v

•

04:44 min

•

February 15, 2022

•

,
1Department of Microbiology, School of Life Science and Technology,China Pharmaceutical University, 2National Experimental Teaching Demonstration Center of Biopharmaceuticals,China Pharmaceutical University

Chapters

  • 00:04Introduction
  • 01:02Preparation of Semisolid Medium
  • 02:01TTC-Enhanced Bacterial Motility Observation
  • 02:57Results: Visualization of Bacterial Motility Using TTC Semisolid Medium
  • 03:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to detect bacterial motility based on a color reaction. Key advantages of this method are that it is easy to evaluate and more accurate, and does not require specialized equipment.

Tags

Bacterial MotilityTTC Semi-solid AgarColor ReactionDehydrogenasesPathogenicityClinical MicrobiologyNon-motile BacteriaMotile BacteriaAgar ConcentrationStaphylococcus AureusEscherichia Coli

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Visualizing RNA Localization in Xenopus Oocytes

Gastrointestinal Motility Monitor (GIMM)

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Ex Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis

Read Article