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Journal
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Biochemistry
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使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段
DOI:
10.3791/63720-v
•
09:20 min
•
April 11, 2022
•
Shawn W. Schowe
,
Conner J. Langeberg
,
Erich G. Chapman
,
Kitty Brown
,
Marino J. E. Resendiz
1
Department of Chemistry
,
University of Colorado, Denver
,
2
Department of Chemistry & Biochemistry
,
University of Denver
,
3
Analytical Resources Core Bioanalysis & Omics
,
Colorado State University
Chapters
00:09
Introduction
01:05
Concentration Determination of RNA Solution
03:15
Hydrolysis of Oligonucleotides of RNA by Xrn-1
05:31
Desalting of RNA Solution, MALDI-TOF Plate Spotting
07:41
Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
08:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
MALDI-TOF用于表征从氧化RNA和外切核糖核酸酶Xrn-1之间的反应性中获得的片段。本方案描述了一种可应用于涉及RNA和/或DNA的其他过程的方法。
Tags
RNA Fragments
Enzymatic Degradation
MALDI-TOF Mass Spectrometry
Biopolymer Characterization
Biochemical Transformations
UV-Vis Spectrophotometry
Peltier Temperature Control
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