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由人诱导多能干细胞改造的同基因肾小球芯片
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
由人诱导多能干细胞改造的同基因肾小球芯片
DOI:
10.3791/63821-v
•
10:23 min
•
November 04, 2022
•
Yasmin Roye
,
Samira Musah
2,3,4
1
Department of Biomedical Engineering, Pratt School of Engineering
,
Duke University
,
2
Department of Medicine, Division of Nephrology
,
Duke University School of Medicine
,
3
Department of Cell Biology
,
Duke University
,
4
Center for Biomolecular and Tissue Engineering
,
Duke University
Chapters
00:04
Introduction
00:59
Preparation of Microfluidic Organ Chip Devices
01:31
Seeding of viECs and Intermediate Mesoderm Cells into the Microfluidic Device
07:15
Podocyte Induction and Chip Maintenance
08:03
Results: Differentiation of viECs and Podocytes on Organ-On-A-Chip Device
09:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一种设计个性化器官芯片系统的协议,该系统通过整合与人类诱导的多能干细胞分化的遗传匹配的上皮和血管内皮细胞来概括肾小球过滤屏障的结构和功能。这种生物工程系统可以推进肾脏精准医疗和相关应用。
Tags
Isogenic Glomerulus Chip
Human Induced Pluripotent Stem Cells
IPSC
Glomerular Epithelium
Glomerular Endothelium
Patient-specific Disease Modeling
Nephrotoxicity Testing
Precision Medicine
Drug Screening
Mechanistic Studies
Body-on-a-chip
Patient-specific Responses
Basement Membrane Matrix
Vascular Endothelial Cells
Microfluidic Chip
PDMS Membrane
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