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Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

DOI:
10.3791/63920-v

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08:31 min

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May 19, 2022

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, ,
1Faculty of Biomedical Engineering,Technion-Israel Institute of Technology

Chapters

  • 00:04Introduction
  • 01:04Vascular Scaffold Fabrication
  • 01:54Granular Support Bath Preparation and Incorporation of Endothelial Cells and Support Cells with the Bioink
  • 03:09Bioprinting Microvascular Networks Using rhCollMA Bioink
  • 06:00Assembling the Bioprinted Microvascular Network with the Vascular Scaffold to Obtain the Engineered Vascularized Flap
  • 07:12Results: Representative Images of the Assembled Vascularized Flap
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.

Tags

Keywords: 3D PrintingVascular FlapsEngineered FlapsHierarchical Vessel NetworksBioprintingBVOH MoldPolymer ScaffoldHAMEC-ZsGreenDental Pulp Stem CellsRhCollMA BioinkCell-laden BioinkMicrosurgical Anastomosis

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