Journal
/
Genetics
/
Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

DOI:
10.3791/63949-v

•

03:45 min

•

August 08, 2022

•

, , , , , , , , ,
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital,Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals,Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Chapters

  • 00:04Introduction
  • 00:35Evaluation of the Cardiac Morphology and Function
  • 01:54Results: Validating the Pathogenicity of G823E Mutation Using the C57BL/6N Murine Model
  • 03:17Conclusion

Summary

Automatic Translation

Automatic Translation

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.

Tags

Keywords: Familial Hypertrophic CardiomyopathyMYH7 MutationGly823GluMouse ModelUltrasoundEchocardiographyCardiac HypertrophySanger SequencingGenetic Etiology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63949?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/63949?article_type=v

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
check_url/63949?article_type=v

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
check_url/63949?article_type=v

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
check_url/63949?article_type=v

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
check_url/63949?article_type=v

Exploring the Effects of Spaceflight on Mouse Physiology using the Open Access NASA GeneLab Platform
check_url/63949?article_type=v

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
check_url/63949?article_type=v

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
check_url/63949?article_type=v

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Read Article