Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Yu Xia; Jinlin Hu; Xiang Li; Shuang Zheng; Ge Wang; Songtao Tan; Zengxiao Zou; Qiong Ling; Fenghua Yang; Xiaoping Fan

doi:10.3791/63949

Journal

/

Genetics

/

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

3,091 Views

•

03:45 min

•

August 08, 2022

DOI:

10.3791/63949-v

DOI:

10.3791/63949-v

•

03:45 min

August 08, 2022

•

3047 Views

Yu Xia*^1,2, Jinlin Hu*³, Xiang Li⁴, Shuang Zheng⁴, Ge Wang^1,2, Songtao Tan^1,2, Zengxiao Zou^1,2, Qiong Ling^2,5, Fenghua Yang⁴, Xiaoping Fan^1,2

¹Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine ²The Second Clinical College of Guangzhou University of Chinese Medicine ³Department of Cardiovascular Surgery, The First Affiliated Hospital,Jinan University ⁴Guangdong Provincial Key Laboratory of Laboratory Animals,Guangdong Laboratory Animals Monitoring Institute ⁵Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Summary

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.

Explore More Videos

MYH7 Mutation Gly823Glu Familial Hypertrophic Cardiomyopathy Mouse Model Ultrasound Technique Pathogenic Genes Cardiac Hypertrophy Echocardiography Sanger Sequencing Heart Rate Measurement Left Ventricular Dimensions Histological Analysis ACMG Guidelines Heterozygous Variant

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Automatically Generated

Summary

Automatically generated

Explore More Videos

Related Videos