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基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物
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Cancer Research
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JoVE Journal Cancer Research
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
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基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物

DOI:
10.3791/63973-v

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08:56 min

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July 12, 2022

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, , , , , ,
1Johannes Kepler University Linz, 2Charité – University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Chapters

  • 00:05Introduction
  • 00:55Stain DLBCL Cells with Different Senescence Markers
  • 04:01Imaging Senescence Markers Using the Imaging Flow Cytometer System
  • 07:12Results: Visualization and Quantification of Senescent Cells on a Single-Cell Level
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种基于流式细胞术的方法，用于可视化和定量单个细胞中多种衰老相关标记物。

Tags

Simultaneous ImagingFlow CytometryFluorescent Senescence MarkersTherapy-induced Senescent Cancer CellsDLBCL Cell LinesEdUChloroquineC12 FDG2-phenylethyl-beta-d-thiogalactosideParaformaldehyde FixationSaponin Permeabilization

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