Journal
/
Developmental Biology
/
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

DOI:
10.3791/64063-v

•

11:41 min

•

June 16, 2022

•

, , ,
1Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences,King’s College London

Chapters

  • 00:05Introduction
  • 00:53Mounting Fish for Confocal Scanning
  • 03:05Confocal Scanning
  • 05:41Optional Method: Remove and Re-Introduce Muscle Electrical Activity
  • 09:01Results: Accurate and Efficient Estimation of the Muscle Volume of Live Zebrafish Larvae at Selected Stages of Activity
  • 11:01Conclusion

Summary

Automatic Translation

Automatic Translation

Optical clarity is a major advantage for cell biological and physiological work in zebrafish. Robust methods for measurement of cell growth in individual animals are described that permit novel insights into how growth of skeletal muscle and neighboring tissues are integrated with whole body growth.

Tags

Keywords: Skeletal Muscle GrowthLive ZebrafishAltered Electrical ActivityIn Vivo MeasurementReal-time MonitoringConfocal MicroscopyLow-melting AgaroseDevelopmental BiologyMuscle Wasting Disorders

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64063?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/64063?article_type=v

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
check_url/64063?article_type=v

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
check_url/64063?article_type=v

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
check_url/64063?article_type=v

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
check_url/64063?article_type=v

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
check_url/64063?article_type=v

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
check_url/64063?article_type=v

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
check_url/64063?article_type=v

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice
check_url/64063?article_type=v

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Read Article