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Levende billeddannelse af mikrotubulusdynamik i glioblastomceller, der invaderer zebrafiskhjernen
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JoVE Journal Biology
Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Levende billeddannelse af mikrotubulusdynamik i glioblastomceller, der invaderer zebrafiskhjernen

DOI:
10.3791/64093-v

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09:29 min

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July 29, 2022

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, ,
1Cell Polarity, Migration and Cancer Unit,Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

Chapters

  • 00:04Introduction
  • 01:03Xenotransplantation Procedure
  • 04:46Intravital Imaging of the Glioblastoma Xenografts
  • 07:41Results: Understanding Microtubule Dynamics of Glioblastoma Cells via Live Imaging of Zebrafish Larval Xenografts
  • 08:53Conclusion

Summary

Automatic Translation

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Vi rapporterer en teknik, der tillader levende billeddannelse af mikrotubulusdynamik i glioblastom (GBM) celler, der invaderer et hvirveldyrs hjernevæv. Kobling af den ortopiske injektion af fluorescerende mærkede GBM-celler til en gennemsigtig zebrafiskhjerne med intravital billeddannelse med høj opløsning muliggør måling af cytoskeletdynamik under in situ kræftinvasion.

Tags

Microtubule DynamicsGlioblastoma CellsZebrafish BrainSubcellular Intravital ImagingBrain Cancer InvasionProtein AnalysisCell TransplantationMicroinjectionAnesthetized Larvae

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