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JoVE Journal Neuroscience
Dissekering av cellautonom funktion av bräckligt X mental retardationsprotein i en hörselkrets genom in ovo-elektroporation
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Dissekering av cellautonom funktion av bräckligt X mental retardationsprotein i en hörselkrets genom in ovo-elektroporation

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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Med hjälp av ovo-elektroporering utarbetade vi en metod för att selektivt transfektera det auditiva innerörat och den cochleära kärnan i kycklingembryon för att uppnå en cellgruppsspecifik knockdown av bräckligt X-mental retardationsprotein under diskreta perioder av kretsmontering.

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Neurovetenskap utgåva 185 bräckligt X-syndrom sensorisk krets innerörat cochleakärna synapsbildning hjärnutveckling endbulb av Held kycklingembryo
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