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Protokolle für CRISPR/Cas9-Mutagenese der Orientalischen Fruchtfliege Bactrocera dorsalis
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Protokolle für CRISPR/Cas9-Mutagenese der Orientalischen Fruchtfliege Bactrocera dorsalis

Article DOI: 10.3791/64195-v • 08:19 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 2, 1, 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Dieser Artikel stellt die Schritt-für-Schritt-Protokolle für die CRISPR/Cas9-Mutagenese der orientalischen Fruchtfliege Bactrocera dorsalis vor. Detaillierte Schritte, die von diesem standardisierten Protokoll bereitgestellt werden, werden als nützlicher Leitfaden für die Erzeugung mutierter Fliegen für funktionelle Genstudien in B. dorsalis dienen.

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Biologie Ausgabe 187
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