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Protocolos para a Mutagênese CRISPR/Cas9 da Mosca da Fruta Oriental Bactrocera dorsalis
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Protocolos para a Mutagênese CRISPR/Cas9 da Mosca da Fruta Oriental Bactrocera dorsalis

Article DOI: 10.3791/64195-v • 08:19 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 2, 1, 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Este trabalho apresenta os protocolos passo-a-passo para a mutagênese CRISPR/Cas9 da mosca da fruta oriental Bactrocera dorsalis. Passos detalhados fornecidos por este protocolo padronizado servirão como um guia útil para a geração de moscas mutantes para estudos de genes funcionais em B. dorsalis.

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Biologia Edição 187
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