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Rekonstituering og karakterisering af actin-mikrotubuli kompositter med indstillelig motordrevet dynamik og mekanik
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Rekonstituering og karakterisering af actin-mikrotubuli kompositter med indstillelig motordrevet dynamik og mekanik

Article DOI: 10.3791/64228-v • 09:10 min • August 25th, 2022
August 25th, 2022 •
1, 1,2, 1, 1, 1, 3, 3, 1, 1
1Department of Physics and Biophysics, University of San Diego, 2W. M. Keck Science Department, Scripps College, Pitzer College, and Claremont McKenna College, 3Department of Physics, Syracuse University

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Dette papir præsenterer protokoller til konstruktion og karakterisering af justerbare tredimensionelle sammensatte netværk af sammenfiltrede actinfilamenter og mikrotubuli. Kompositter gennemgår aktiv omstrukturering og ballistisk bevægelse, drevet af myosin II og kinesinmotorer, og er indstillet af de relative koncentrationer af actin, mikrotubuli, motorproteiner og passive tværbindinger.

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Biologi udgave 186 cytoskelet aktivt stof actin mikrotubuli myosin kinesin kompositter biopolymerer in vitro-rekonstitution fluorescenskonfokal mikroskopi
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