Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

Flash-and-Freeze
 

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Article DOI: 10.3791/55664 10:01 min
May 1st, 2017

Chapters

Summary May 1st, 2017

We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter