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JoVE Journal

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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Article DOI: 10.3791/55664 10:01 min
May 1st, 2017


Summary May 1st, 2017

We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.


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