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Journal
/
Neuroscience
/
網膜組織における免疫染色カスパーゼ-9の定量
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Quantification of Immunostained Caspase-9 in Retinal Tissue
Please note that all translations are automatically generated.
Click here for the English version.
網膜組織における免疫染色カスパーゼ-9の定量
DOI:
10.3791/64237-v
•
05:18 min
•
July 25, 2022
•
Crystal K. Colón Ortiz
,
Anna M. Potenski
,
Kendra V. Johnson
,
Claire W. Chen
,
Scott J. Snipas
,
Ying Y. Jean
,
Maria I. Avrutsky*
1
,
Carol M. Troy*
1,4,5
1
Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons
,
Columbia University
,
2
Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons
,
Columbia University
,
3
NCI-designated Cancer Center
,
Sanford Burnham Prebys Medical Discovery Institute
,
4
Department of Neurology, Vagelos College of Physicians and Surgeons
,
Columbia University
,
5
The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons
,
Columbia University
Chapters
00:05
Introduction
00:48
Quantification of Caspase Levels
03:21
Results: Analyze and Quantify Caspase-9 Levels in the Retinal Tissue
04:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、複雑な組織における機能的に関連するカスパーゼを同定、検証、標的化するための詳細な免疫組織化学プロトコルを示します。
Tags
Immunohistochemistry
Caspase-9
Confocal Microscopy
Quantification
Apoptosis
Cell Biology
Therapeutic Targets
Disease
Z Stack
Brightness And Contrast
Colocalization
Vascular Marker
Neuronal Areas
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