/

Immunology and Infection

/

肠道细菌侵入肠上皮细胞<em>体外</em>使用垂直扩散室模型，显着增强

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

Please note that all translations are automatically generated. Click here for the English version.

肠道细菌侵入肠上皮细胞<em>体外</em>使用垂直扩散室模型，显着增强

DOI:

10.3791/50741-v

•

10:52 min

•

October 22, 2013

•

Neveda Naz, Dominic C. Mills, Brendan W. Wren, Nick Dorrell

¹Faculty of Infectious & Tropical Diseases,London School of Hygiene & Tropical Medicine

Chapters

00:05Title
01:47Growth of Intestinal Epithelial Cell (IEC) Monolayers on 0.4 µm Filters
02:22Vertical Diffusion Chamber (VDC) Co-culture Assay Preparation
04:21Setting up of the VDC
06:14Disassembling of the VDC after Co-culture
08:34Results: The VDC Model Facilitates Greater Numbers of Bacterial-IEC Interactions and Invasions than Standard Cell Culture Assay Conditions
10:07Conclusion

Summary

Automatic Translation

在有氧条件下进行细胞培养实验调查细菌的粘附和侵袭，通常是不具代表性的<em>在体内</em>环境。 A垂直扩散室模型允许人类病原体的相互作用研究<em>空肠弯曲菌</em与肠上皮细胞在更<em>在体内</em>如条件，从而增强细菌的侵袭。

Tags

Keywords: Vertical Diffusion Chamber In Vitro Model Intestinal Epithelial Cells Campylobacter Jejuni Microaerobic Conditions Host-pathogen Interactions Bacterial Invasion Cell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在体外生物膜的形成以及8商会幻灯片

由细菌病原体入侵人体细胞

到哺乳动物的上皮细胞在体外细菌粘附的含量

鼠标应用结扎Peyer氏修补肠袢含量评价M细胞的细菌吸收

使用人类肠道上皮细胞和BALB / C小鼠巨噬细胞的容忍脂肪酸和霍乱毒素浓度的测定

体外共生分析评估病原体诱导中性粒细胞跨上皮迁移

使用李斯特菌单细胞基因评估受感染小鼠培养和心脏殖民化中心脏细胞的细菌入侵

哺乳动物细胞细菌挑战后应激颗粒形成的免疫荧光分析

一种高通量志贺氏菌特异性杀菌试验

利用人类诱导的多能干细胞衍生的肠道有机体研究和改性上皮细胞对沙门氏菌和其他病原体的保护

Read Article