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Journal
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Immunology and Infection
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接触超敏反应作为过敏性接触性皮炎的小鼠模型
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Immunology and Infection
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Immunology and Infection
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
Please note that all translations are automatically generated.
Click here for the English version.
接触超敏反应作为过敏性接触性皮炎的小鼠模型
DOI:
10.3791/64329-v
•
08:25 min
•
September 26, 2022
•
Magdalena Zemelka-Wiacek
,
Monika Majewska-Szczepanik
,
Pawel Gajdanowicz
,
Marian Szczepanik
1
Department of Clinical Immunology
,
Wroclaw Medical University
,
2
Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences
,
Jagiellonian University Medical College
,
3
Chair of Biomedical Sciences, Faculty of Health Sciences
,
Jagiellonian University Medical College
Chapters
00:04
Introduction
00:59
Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)
02:19
Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay
03:48
Vascular Permeability Test
04:43
Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement
05:16
Adoptive Transfer of the CHS-Effector Cells
06:41
Results: Study of the Contact Hypersensitivity Reaction in Mice
07:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
接触性超敏反应(CHS)是过敏性接触性皮炎(ACD)的小鼠实验模型。CHS基于通过涂上胸部和腹部的剃须皮肤对反应性半抗原的致敏,随后用稀释的半抗原进行耳朵皮肤激发,引起肿胀反应,以各种方式评估。
Tags
Contact Hypersensitivity
Allergic Contact Dermatitis
Mouse Model
Ear Edema
T-cell Dependent Immune Response
Hapten
Myeloperoxidase (MPO) Assay
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