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Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
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JoVE Journal Cancer Research
Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

DOI:
10.3791/64632-v

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05:46 min

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December 02, 2022

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, , , , , , ,
1Ophthalmology Research Laboratory,Kaplan Medical Center, 2Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine,The Hebrew University of Jerusalem

Chapters

  • 00:05Introduction
  • 01:23Animal Preparation and Creating Conjunctival Peritomy
  • 02:31Inoculation of Melanoma Cells
  • 03:02Assessing the Location of the Injection and Predicting Tumor Size Based on Retinal Detachment (RD) Height
  • 03:43Results: OCT-Based Prediction of Tumor Growth Following Sub-Choroidal Cell Injection and Classification of Tumor Size
  • 05:08Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the implantation and evaluation of melanoma in the murine choroid utilizing optical coherence tomography.

Tags

Keywords: MelanomaChoroidalOptical Coherence TomographyTumor InductionMouse ModelUveal MelanomaTumor ProgressionTumor Cell InoculationLive ImagingScleral Injection

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