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Journal
/
Medicine
/
全細胞パッチクランプ法
による
H9c2心筋細胞の電位依存性カリウム電流記録
JoVE Journal
Medicine
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JoVE Journal
Medicine
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes
via
the Whole-Cell Patch-Clamp Technique
Please note that all translations are automatically generated.
Click here for the English version.
全細胞パッチクランプ法
による
H9c2心筋細胞の電位依存性カリウム電流記録
DOI:
10.3791/64805-v
•
08:11 min
•
November 11, 2022
•
Hong Jiang*
1
,
Yating Zhang*
1
,
Ya Hou
,
Lijun Li
,
Sanyin Zhang
4
,
Yi Zhang
,
Xianli Meng
3,4
,
Xiaobo Wang
1
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy
,
Chengdu University of Traditional Chinese Medicine
,
2
Chengdu Techman Instrument Co., Ltd.
,
3
Innovative Institute of Chinese Medicine and Pharmacy
,
Chengdu University of Traditional Chinese Medicine
,
4
Research Institute of Integrated TCM & Western Medicine
,
Chengdu University of Traditional Chinese Medicine
,
5
School of Ethnic Medicine
,
Chengdu University of Traditional Chinese Medicine
Chapters
00:04
Introduction
01:04
Cell Culture
01:41
Fabrication of Micropipettes
02:16
Instrument Setup
02:37
I
Kv
Parameter Setting
03:16
Whole‐Cell Patch‐Clamp Recording of the I
Kv
in Voltage‐Clamp Mode
06:43
Results: I
Kv
in H9c2 Cells
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本プロトコルは、全細胞パッチクランプ技術を使用して、H9c2心筋細胞における電位依存性カリウム(Kv)チャネル電流をリアルタイムかつ動的に取得するための効率的な方法を記載しています。
Tags
Voltage-dependent Potassium Current
Whole-cell Patch-clamp Technique
H9C2 Cardiomyocytes
Ion Channel Research
Pharmacological Mechanisms
Cardiotoxicity
Micropipette Puller
Borosilicate Glass Capillary
Digital Analog Converter
Signal Amplifier
Micro Manipulator
Data Acquisition Software
Leak Subtraction
Membrane Test
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