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Journal / Neuroscience / In vivo Kalsiumavbildning av nevronale ensembler…
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Neuroscience

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JoVE Journal Neuroscience
In vivo Kalsiumavbildning av nevronale ensembler i nettverk av primære sensoriske nevroner i intakte dorsale rotganglier
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In vivo Kalsiumavbildning av nevronale ensembler i nettverk av primære sensoriske nevroner i intakte dorsale rotganglier

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Denne protokollen beskriver kirurgisk eksponering av dorsalrotganglion (DRG) etterfulgt av GCaMP3 (genetisk kodet Ca2+ indikator; Grønt fluorescerende protein-Calmodulin-M13 Protein 3) Ca2+ avbildning av nevronensemblene ved bruk av Pirt-GCaMP3-mus mens du bruker en rekke stimuli til den ipsilaterale bakpoten.

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