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JoVE Journal Neuroscience
In vivo Imágenes de calcio de conjuntos neuronales en redes de neuronas sensoriales primarias en ganglios intactos de la raíz dorsal
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In vivo Imágenes de calcio de conjuntos neuronales en redes de neuronas sensoriales primarias en ganglios intactos de la raíz dorsal

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Este protocolo describe la exposición quirúrgica del ganglio de la raíz dorsal (GRD) seguida de GCaMP3 (indicador de Ca2+ codificado genéticamente; Proteína fluorescente verde-Calmodulina-M13 Proteína 3) Imagen de Ca2+ de los conjuntos neuronales utilizando ratones Pirt-GCaMP3 mientras se aplica una variedad de estímulos a la pata trasera ipsilateral.

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Este mes en JoVE Número 192
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