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In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier

DOI:
10.3791/64826-v

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09:07 min

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February 10, 2023

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, , , ,
1Department of Oral & Maxillofacial Surgery, School of Dentistry,University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences,University of Texas Health Science Center at San Antonio

Chapters

  • 00:04Introduction
  • 01:09Surgery and Securing the Animal for Right Side L5 DRG Imaging
  • 03:08DRG Imaging
  • 07:02Results: Imaging Neuronal Population Using Confocal Scanning of Pirt-GCaMP L5 DRG
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver den kirurgiske eksponering af dorsalrodsganglion (DRG) efterfulgt af GCaMP3 (genetisk kodet Ca2+ indikator; Grønt fluorescerende protein-calmodulin-M13-protein 3) Ca2+ billeddannelse af neuronale ensembler ved hjælp af Pirt-GCaMP3-mus, mens der påføres en række stimuli på den ipsilaterale bagpote.

Tags

Keywords: In Vivo Calcium ImagingNeuronal EnsemblesPrimary Sensory NeuronsDorsal Root GangliaPeripheral AllodyniaPain HypersensitivityTrigeminal GangliaGeniculate GangliaGenetically Encoded SensorsVoltageCell Signaling MoleculesCyclic AMPSurgical ProcedureLumbar EnlargementTransverse ProcessL5 DRGIsoflurane Anesthesia

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