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Journal / Neuroscience / In vivo Imaging del calcio di insiemi neuronali…
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JoVE Journal Neuroscience
In vivo Imaging del calcio di insiemi neuronali in reti di neuroni sensoriali primari nei gangli della radice dorsale intatti
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In vivo Imaging del calcio di insiemi neuronali in reti di neuroni sensoriali primari nei gangli della radice dorsale intatti

Article DOI: 10.3791/64826-v • 09:07 min • February 10th, 2023
February 10th, 2023 •
1, 1, 1, 1, 1,2
1Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio

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Questo protocollo descrive l'esposizione chirurgica del ganglio della radice dorsale (DRG) seguito da GCaMP3 (indicatore Ca2+ codificato geneticamente; Green Fluorescent Protein-Calmodulin-M13 Protein 3) Ca2+ imaging degli insiemi neuronali utilizzando topi Pirt-GCaMP3 mentre si applica una varietà di stimoli alla zampa posteriore omolaterale.

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Questo mese in JoVE numero 192
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