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电泳分离蛋白质
JoVE Journal
Biology
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JoVE Journal
Biology
Electrophoretic Separation of Proteins
Please note that all translations are automatically generated.
Click here for the English version.
电泳分离蛋白质
DOI:
10.3791/758-v
•
08:17 min
•
June 12, 2008
•
Bulbul Chakavarti
,
Deb Chakavarti
1
Proteomic Center
,
Keck Graduate Institute of Applied Life Sciences
Chapters
00:00
Title: Electrophoretic
00:45
Introduction
01:06
Pour the Separating Gel
02:44
Pour The Stacking Gel
03:55
Prepare The Sample and Load the Gel
06:24
Run the Gel
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这段视频中,我们展示了使用acrylimide聚凝胶电泳(PAGE)蛋白电泳分离的方法。
Tags
Electrophoretic Separation
Proteins
Protein Separation
Electrophoresis
Complex Mixtures
Subunit Compositions
Protein Samples
Purification Of Proteins
Polyacrylamide Gel Electrophoresis
Electrical Field
Pore Size
Acrylamide Concentration
Protein Charge
Protein Size
Protein Shape
Migration Rate
Laemmli Method
Discontinuous Gel Electrophoresis
Denaturing Conditions
Sodium Dodecyl Sulfate (SDS)
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