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In-vitro-Differenzierung von embryonalen Stammzellen der Maus (mES) Zellen unter Verwendung des Hanging-Drop-Methode
JoVE Journal
Biology
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JoVE Journal Biology
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
DOI:

07:07 min

July 23, 2008

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Chapters

  • 00:00Title
  • 01:10Introduction
  • 02:06Preparing Single Cell Suspension of Embryonic Stem Cells
  • 03:46Generating Hanging Drop Cultures
  • 04:39Transferring Hanging Drops to Ultralow Attachment Plates
  • 05:17Plating Embryoid Bodies onto Gelatin- Coated Plates
  • 06:12Conclusion

Summary

Automatic Translation

Dieses Video zeigt, wie in vitro-Differenzierung von embryonalen Stammzellen der Maus, um Embryoidkörpern mit dem hängenden Tropfen-Methode durchzuführen.

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