JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
产生稳定的转基因C.使用微量注射线虫
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Generation of Stable Transgenic C. elegans Using Microinjection
Please note that all translations are automatically generated.
Click here for the English version.
产生稳定的转基因C.使用微量注射线虫
DOI:
10.3791/833-v
•
12:09 min
•
August 15, 2008
•
Laura A. Berkowitz
,
Adam L. Knight
,
Guy A. Caldwell
,
Kim A. Caldwell
1
Department of Biological Sciences
,
University of Alabama
Chapters
00:00
Title
00:47
Introduction
01:31
Preparing for Microinjection
04:38
Setting Up the Microinjection Microscope
07:16
Injecting Expression Vector Constructs into C. elegans
10:31
Screening for Transgenic C. elegans
11:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本视频演示,到线虫的性腺创建转基因动物的显微注射技术。
Tags
Transgenic C. Elegans
Microinjection
DNA Plasmid
Concatamers
Phenotypic Marker
GFP
Cellular Localization Studies
Gene Expression
Germline
Early Embryo
Experimental Paradigms
Transgenic Worms
Selection
Maintenance
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
埃及斑蚊胚胎显微注射获得转基因蚊子
生成的基因
C。线虫
通过基因枪转化
成像
C。线虫
胚胎使用啶显微镜和开放源码软件
激活细胞凋亡的细胞色素的细胞质显微注射
C</em
确定基因表达概况
C。线虫
使用微阵列和实时PCR
线虫
趋含量的
抗体在染色<em> C。线虫</em>使用“冻结开裂”
测量细菌的影响<em> C。线虫</em>行为使用一个鸡蛋保留含量
转基因的产生<em>水润</em>通过胚胎显微注射
调查的传播和毒性朊病毒样蛋白使用后生动物模式生物<em>温度。线虫</em
Read Article