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Studera Membran biogenes med en luciferas-Based Reporter Gene Assay
JoVE Journal
Biology
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JoVE Journal Biology
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
DOI:

13:48 min

September 07, 2008

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Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:21Preparing Transfections
  • 05:35Inducing Membrane Biosynthesis via Phagocytosis of Latex Beads
  • 08:07Performing the Dual-Glo Luciferase Assays
  • 11:28Data Analysis
  • 13:00Conclusion

Summary

Automatic Translation

Här beskriver vi rutiner för att studera förändringar i fagocytos-inducerad genuttryck med en luciferas-baserad reporter gen metod att använda Dual-GloTM luciferas analyssystem från Promega.

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