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研究与基于荧光素酶的记者基因检测膜生源
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Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Please note that all translations are automatically generated.
Click here for the English version.
研究与基于荧光素酶的记者基因检测膜生源
DOI:
10.3791/920-v
•
13:48 min
•
September 07, 2008
•
Shaochong Zhang
,
Axel Nohturfft
2
1
Department of Molecular and Cell Biology
,
Harvard
,
2
Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences
,
St. George’s University of London
Chapters
00:00
Title
00:49
Introduction
01:21
Preparing Transfections
05:35
Inducing Membrane Biosynthesis via Phagocytosis of Latex Beads
08:07
Performing the Dual-Glo Luciferase Assays
11:28
Data Analysis
13:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述基于荧光素酶报告基因的使用自Promega的双GloTM荧光素酶检测系统的方法研究细胞的吞噬功能,诱导基因表达的变化的过程。
Tags
Membrane Biogenesis
Luciferase-based Reporter Gene Assay
Lipid Synthesis Pathways
Experimental System
Phagocytosis
Latex Beads
Membrane Lipids
Gene Expression
Dual-Glo Luciferase Assay System
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