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Making Gynogenetic Diploid Zebrafish by Early Pressure
 

Making Gynogenetic Diploid Zebrafish by Early Pressure

Article DOI: 10.3791/1396-v 12:41 min June 30th, 2009
June 30th, 2009

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Summary

This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.

Tags

Gynogenetic Diploid Zebrafish Early Pressure Method Homozygous Offspring Heterozygous Females F1 Mutagenized Females Forward Genetic Screen Ultraviolet Light-inactivated Sperm Physical Treatments Heat Treatment Pressure Treatment Microtubules Meiosis II Viable Embryos Fertile Adults Loci Centromere Recombination EP Clutches Centromeric Loci Telomeric Loci Zebrafish Book
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