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Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
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Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

DOI:
10.3791/2144-v

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08:19 min

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September 04, 2010

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,
1Department of Chemistry,George Washington University

Chapters

  • 00:00Title
  • 01:06Introduction
  • 01:34Visualization System
  • 03:27Electrospray Setup
  • 04:27Sample Preparation
  • 05:17Acquisition of Mass Spectra
  • 06:46Representative Results
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Single cell analysis is performed by mass spectrometry on plant and animal cells at atmospheric pressure by using a sharpened optical fiber to sample the cells for laser ablation electrospray ionization (LAESI) mass spectrometry.

Tags

Single CellsMass SpectrometryAtmospheric PressureBiochemical AnalysisCell MetabolismCell CycleAdaptationDisease StatesHeterogeneous Biochemical MakeupSample PreparationAmbient Ionization MethodsMid Infrared Laser AblationBiofluidsLAESIAP IR-MALDI

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