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Journal
/
Biology
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Single-Molecule Imaging of Nuclear Transport
JoVE Journal
Biology
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JoVE Journal
Biology
Single-Molecule Imaging of Nuclear Transport
Single-Molecule Imaging of Nuclear Transport
DOI:
10.3791/2040-v
•
12:13 min
•
June 09, 2010
•
Alexander Goryaynov
,
Ashapurna Sarma
,
Jiong Ma
,
Weidong Yang
2
1
Department of Biology
,
Bowling Green State University
,
2
Center for Photochemical Sciences
,
Bowling Green State University
Chapters
00:00
Title
00:41
Introduction
01:20
Preparation of Essential Proteins for Nuclear Transport Experiment
06:10
Preparation of the Cell System for Single Molecule Experiment
08:52
Single Molecule Microscopy
10:22
Representative Single Molecule Imaging
11:39
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
Single molecule microscopy approch provided novel insights into nuclear transport.
Tags
Single-molecule Imaging
Nuclear Transport
Single Molecule Fluorescence Microscopy
Biological Reactions
Molecular Interactions
High Temporal Resolution
High Spatial Resolution
Narrow-field Epifluorescence Microscopy
Nucleocytoplasmic Transport
Single Molecule Approach
Kinetics
Nuclear Transport Time
Efficiency
Signal-dependent Cargo Molecules
Signal-independent Cargo Molecules
Improved Spatiotemporal Resolution
Transient Active Transport
Passive Diffusion Events
Nuclear Pore Complexes (NPC)
Semi-intact Cells
Binding Events
Trafficking Events
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