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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
DOI:

10:55 min

December 07, 2010

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Chapters

  • 00:01Title
  • 02:53Primary and Secondary Antibodies
  • 09:35Imaging and Analysis

Summary

Automatic Translation

We have derived a strategy to detect sequential incorporation of thymidine analogues (CldU and IdU) into tissues of adult mice to quantify two successive rounds of cell division. This strategy is useful to detect cell turnover of long-lived tissues, oncogenic transformation, or transit-amplifying cells.

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