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Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
JoVE Journal
Biology
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JoVE Journal Biology
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
DOI:

07:34 min

April 24, 2011

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Chapters

  • 00:05Title
  • 01:34Cell Preparation
  • 02:14Application of Annexin V/PI Stain
  • 04:26RNase-treated Cells and Cell Lines Exhibit Fewer False Positive Staining Results
  • 06:56Conclusion

Summary

Automatic Translation

An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.

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