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A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
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1Institute for BioNanotechnology in Advanced Medicine,Northwestern University, 2Department of Obstetrics and Gynecology,Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research,Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center,Northwestern University, 5Department of Chemical and Biological Engineering,Northwestern University
Chapters
- 00:05Title
- 03:02Follicle Isolation
- 05:11The Drop Method
- 07:16The Parafilm Method
- 08:31Follicle Recovery from the 3D Matrix
- 10:00Alginate and Fibrin-alginate for 3D Follicle Culture in Vitro
- 11:48Conclusion
A new method for ovarian follicle encapsulation in a 3D fibrin-alginate interpenetrating network is described. This system combines structural support with proteolytic degradation to support the development of immature follicles to produce mature oocytes. This method may be applied to culture cell aggregates to maintain cell-cell contacts without limiting expansion.
Tags
Ovarian FollicleEncapsulationCultureProteolytically Degradable3 Dimensional SystemIn Vitro Follicle TechniquesHydrogelsNative Ovarian Environment3D Follicular ArchitectureCell-cell InteractionsParacrine SignalingAlginateInert Algae-derived PolysaccharideGelationCalcium IonsDevelopmental CompetenceDegradableFibrin-alginate Interpenetrating Network (FA-IPN)Dynamic Mechanical Environment
