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Performing Custom MicroRNA Microarray Experiments
JoVE Journal
Biology
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JoVE Journal Biology
Performing Custom MicroRNA Microarray Experiments
DOI:

07:04 min

October 28, 2011

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Chapters

  • 00:05Title
  • 01:10Printing Custom miRNA Microarrays and Sample Preparation
  • 02:23Microarray Hybridization
  • 04:32Post Hybridization Processing
  • 06:03Representative Microarray Results
  • 06:39Conclusion

Summary

Automatic Translation

A simple procedure of performing custom microRNA microarray experiments is described. The steps include isolating RNA, labeling RNA and reference DNA, hybridizing the samples to microarrays, scanning the microarrays, quantifying and analyzing hybridization signals.

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