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Performing Custom MicroRNA Microarray Experiments
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JoVE Journal Biology
Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

DOI:
10.3791/3250-v

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07:04 min

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October 28, 2011

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,
1Department of Pharmacology,University of Minnesota , 2Masonic Cancer Center,University of Minnesota

Chapters

  • 00:05Title
  • 01:10Printing Custom miRNA Microarrays and Sample Preparation
  • 02:23Microarray Hybridization
  • 04:32Post Hybridization Processing
  • 06:03Representative Microarray Results
  • 06:39Conclusion

Summary

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A simple procedure of performing custom microRNA microarray experiments is described. The steps include isolating RNA, labeling RNA and reference DNA, hybridizing the samples to microarrays, scanning the microarrays, quantifying and analyzing hybridization signals.

Tags

Custom MicroRNA MicroarrayMiRNAsRNA MoleculesTarget GenesPost-transcriptionallyBiological ProcessesAltered MiRNA ExpressionHuman DiseasesBiomarkersPrognosisMiRNA Expression ProfilingReal-time PCRDeep SequencingMiRNA Microarray TechniqueHigh-throughputLess ExpensiveMolecular Biology LaboratoryUniversitiesMedical SchoolsHospitalsCustom MiRNA Microarray ExperimentsGlass SlidesRNA IsolationFluorescence Dye

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