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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
JoVE Journal
Biology
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JoVE Journal Biology
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
DOI:

09:40 min

February 13, 2012

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Chapters

  • 00:05Title
  • 01:24Preparation of Bacteria with Library Clones
  • 03:14Preparation of Nematodes
  • 06:51Observation of Nematodes
  • 08:55Conclusion

Summary

Automatic Translation

We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.

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