Journal
/
Immunology and Infection
/
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

DOI:
10.3791/3697-v

•

10:29 min

•

August 29, 2012

•

,
1Department of Surgery,The Ohio State University Medical Center

Chapters

  • 00:05Title
  • 01:10Lethal Irradiation and Splenocyte Preparation
  • 03:12Bone Marrow Preparation and Removal
  • 05:16CD3 Depletion
  • 06:08L2G85.B6 (LUX+) CD8+ T Cell Purification and Injection Preparation
  • 07:48Bioluminescent Imaging
  • 08:12Results
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

Murine bone marrow transplantation is a widely used technique to study immunological mechanisms governing graft-versus-host disease in humans. The ability to monitor T cell trafficking patterns in vivo allows for detailed analysis of the development and perpetuation of T cell responses during graft-versus-host disease.

Tags

Graft-versus-host DiseaseBone Marrow TransplantHematological DeficienciesAlloreactive T CellsBioluminescent ImagingT Cell TraffickingL2G85.B6 Transgenic MiceLuciferaseSpecific T Cell SubsetsGVHD PromotionTime PointsXenogen IVIS CCD CameraLiving Image Software

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Retroviral Transduction of T-cell Receptors in Mouse T-cells

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Read Article