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Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
DOI:

11:29 min

October 03, 2012

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Chapters

  • 00:05Title
  • 02:02Preparation of Brain Homogenate and Detergent Soluble and Insoluble Fractions
  • 03:21Velocity Sedimentation in Sucrose Step Gradients
  • 04:30Size Exclusion Chromatography
  • 06:16Capture of PrP with g5P and Western Blotting
  • 08:20Representative PrP Isolation Results
  • 10:45Conclusion

Summary

Automatic Translation

A new species of cellular prion protein (PrPC) has recently been identified in uninfected human brains using the methods described here. These methods can be used to isolate various PrP species, while some of them are also useful in isolating other misfolded protein aggregates from human brains.

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