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Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
DOI:

11:01 min

January 11, 2012

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Chapters

  • 00:05Title
  • 01:09Culture of hNPCs in a PuraMatrix
  • 03:41Immunocytochemical Staining of Entire Matrices
  • 05:44Release of hNPCs from the Scaffolds for Flow-cytometry Analysis
  • 07:04Quantification of βIII-tubulin Positive Cells by Flow Cytometry
  • 08:09Observation and Enumeration of PuraMatrix Encapsulated hNPCs
  • 10:37Conclusion

Summary

Automatic Translation

Here we describe the use of a self-assembling 3-dimensional scaffold to culture human neural progenitor cells. We present a protocol to release the cells from the scaffolds to be analysed subsequently e.g. by flow cytometry. This protocol might be adapted to other cell types to perform detailed mechanistically studies.

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