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Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
JoVE Journal
Medicine
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JoVE Journal Medicine
Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
DOI:

10:40 min

August 04, 2012

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Chapters

  • 00:05Title
  • 02:44Labeling Erythrocytes with Fluorescein Isothiocyanate Isomer (FITC)
  • 04:11Preparation of Oxygen-sensitive Dye
  • 04:54Animal Preparation
  • 05:58Blood Flow Visualization
  • 06:15pO2 Measurement
  • 08:04Hepatitis Model
  • 08:31Representative Results
  • 10:12Conclusion

Summary

Automatic Translation

An optical system was developed to visualize hepatic microcirculation with FITC-labeled erythrocytes and to measure the partial pressure of oxygen in the microvessels with laser-assisted phosphorimetry. This method can be used to investigate physiological and pathological mechanisms by analyzing microvascular structure, diameter, blood flow velocity, and oxygen tension.

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