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Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
DOI:

09:47 min

November 01, 2007

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Chapters

  • 00:00Title
  • 00:16Introduction
  • 01:58Preparation of Cell Suspension from Lymph Nodes
  • 04:04Erythrocyte Lysis
  • 06:13Magnetic Labelling of Cells and Setting up MACS Column
  • 07:49Separation of CD4+ T cells using MACs Column
  • 09:13Conclusion

Summary

Automatic Translation

Isolation of lymphocytes using the Miltenyi MACs kit is a reliable way to purify cells from whole lymphoid tissue homogenates. Cells purified using the Miltenyi system are typically ≥ 96% pure. Here, we demonstrate the steps taken to isolate CD4+ T cells, one of the many kits offered by Miltenyi.

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