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Chapters
- 00:05Title
- 01:39Preparation of DNA, Solutions, and Glass Pipettes
- 02:54Animal Preparation, Glass Pipette Loading and Plasmid Injection
- 05:04Electroporation and Recovery
- 06:44Results: Images of an Electroporated Subventricular Zone
- 07:48Conclusion
We demonstrate a minimally invasive technique referred to as neonatal subventricular zone electroporation. The technique consists of injecting plasmid DNA into the lateral ventricles of neonatal pups and applying electrical current to deliver and genetically manipulate neural stem cells
