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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
DOI:

10:49 min

April 14, 2013

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Chapters

  • 00:05Title
  • 01:39Generation of Human Pluripotent Stem Cell Aggregates
  • 03:53Induction of Neuroepithelial Cells
  • 05:11Generation of Telencephalic Progenitors
  • 06:33Differentiation into Telencephalic Neurons
  • 08:18Telencephalic Neuronal Generation
  • 10:11Conclusion

Summary

Automatic Translation

This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

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