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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

DOI:
10.3791/50488-v

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10:16 min

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February 08, 2014

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, ,
1The Rowland Institute,Harvard University, 2Faculdade de Ciências e Letras de Assis,Universidade Estadual Paulista

Chapters

  • 00:05Title
  • 00:57Bacterial Sample Preparation
  • 02:30Image Acquisition
  • 04:28Reconstruction
  • 07:53Rendering
  • 09:25Results: Digital Inline Holographic Imaging
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.

Tags

Digital Inline Holographic MicroscopyWeakly-scattering ObjectsPhase ContrastDIC3D ReconstructionRayleigh-Sommerfeld Back PropagationGouy Phase AnomalyCMOSCCDLEDLaser Illumination

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